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primary human follicle dp cells hfdpcs  (PromoCell)


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    PromoCell primary human follicle dp cells hfdpcs
    (A) Schematic of the spheroid invasion assay. DP spheroids, each composed of 300 <t>HFDPCs,</t> were placed on an ECM gel layer and overlaid with an additional ECM gel layer. The spheroids <t>were</t> <t>cultured</t> and observed every 12 hours. (B) Phase-contrast images of DP spheroids cultured in collagen I gel (top) and Matrigel (bottom). The colored squares indicate regions magnified in the bottom right images. Scale bar: 100 µm. (C) Binary-masked images highlighting the spheroid area. (D) Line graphs showing the changes in spheroid area over time in collagen I gel (red) and Matrigel (green). (E) Box plots showing the relative spheroid area at 12 h, normalized to the initial time point (1 h). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Two-tailed unpaired t -tests were used: * p <0.05, ** p <0.01, and *** p <0.001. n = 9 wells. (F and G) Box plots showing the relative spheroid area with integrin blocking antibodies in collagen I gel (F) and Matrigel (G). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Statistical comparisons were performed using the Kruskal‒Wallis test followed by Dunn’s post hoc test. Adjusted p -values from the Benjamini‒Hochberg method are shown: * p <0.05, ** p <0.01, *** p <0.001. n = 9 wells pooled from 3 independent experiments.
    Primary Human Follicle Dp Cells Hfdpcs, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+human+follicle+dermal+papilla+cells/bio_rxiv__2025__08__27__672757-185-0-9?v=PromoCell
    Average 95 stars, based on 125 article reviews
    primary human follicle dp cells hfdpcs - by Bioz Stars, 2026-07
    95/100 stars

    Images

    1) Product Images from "Basement membrane components define the microenvironment of aggregated fibroblasts in the skin and support their aggregation in vitro"

    Article Title: Basement membrane components define the microenvironment of aggregated fibroblasts in the skin and support their aggregation in vitro

    Journal: bioRxiv

    doi: 10.1101/2025.08.27.672757

    (A) Schematic of the spheroid invasion assay. DP spheroids, each composed of 300 HFDPCs, were placed on an ECM gel layer and overlaid with an additional ECM gel layer. The spheroids were cultured and observed every 12 hours. (B) Phase-contrast images of DP spheroids cultured in collagen I gel (top) and Matrigel (bottom). The colored squares indicate regions magnified in the bottom right images. Scale bar: 100 µm. (C) Binary-masked images highlighting the spheroid area. (D) Line graphs showing the changes in spheroid area over time in collagen I gel (red) and Matrigel (green). (E) Box plots showing the relative spheroid area at 12 h, normalized to the initial time point (1 h). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Two-tailed unpaired t -tests were used: * p <0.05, ** p <0.01, and *** p <0.001. n = 9 wells. (F and G) Box plots showing the relative spheroid area with integrin blocking antibodies in collagen I gel (F) and Matrigel (G). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Statistical comparisons were performed using the Kruskal‒Wallis test followed by Dunn’s post hoc test. Adjusted p -values from the Benjamini‒Hochberg method are shown: * p <0.05, ** p <0.01, *** p <0.001. n = 9 wells pooled from 3 independent experiments.
    Figure Legend Snippet: (A) Schematic of the spheroid invasion assay. DP spheroids, each composed of 300 HFDPCs, were placed on an ECM gel layer and overlaid with an additional ECM gel layer. The spheroids were cultured and observed every 12 hours. (B) Phase-contrast images of DP spheroids cultured in collagen I gel (top) and Matrigel (bottom). The colored squares indicate regions magnified in the bottom right images. Scale bar: 100 µm. (C) Binary-masked images highlighting the spheroid area. (D) Line graphs showing the changes in spheroid area over time in collagen I gel (red) and Matrigel (green). (E) Box plots showing the relative spheroid area at 12 h, normalized to the initial time point (1 h). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Two-tailed unpaired t -tests were used: * p <0.05, ** p <0.01, and *** p <0.001. n = 9 wells. (F and G) Box plots showing the relative spheroid area with integrin blocking antibodies in collagen I gel (F) and Matrigel (G). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Statistical comparisons were performed using the Kruskal‒Wallis test followed by Dunn’s post hoc test. Adjusted p -values from the Benjamini‒Hochberg method are shown: * p <0.05, ** p <0.01, *** p <0.001. n = 9 wells pooled from 3 independent experiments.

    Techniques Used: Invasion Assay, Cell Culture, Two Tailed Test, Blocking Assay



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    (A) Schematic of the spheroid invasion assay. DP spheroids, each composed of 300 <t>HFDPCs,</t> were placed on an ECM gel layer and overlaid with an additional ECM gel layer. The spheroids <t>were</t> <t>cultured</t> and observed every 12 hours. (B) Phase-contrast images of DP spheroids cultured in collagen I gel (top) and Matrigel (bottom). The colored squares indicate regions magnified in the bottom right images. Scale bar: 100 µm. (C) Binary-masked images highlighting the spheroid area. (D) Line graphs showing the changes in spheroid area over time in collagen I gel (red) and Matrigel (green). (E) Box plots showing the relative spheroid area at 12 h, normalized to the initial time point (1 h). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Two-tailed unpaired t -tests were used: * p <0.05, ** p <0.01, and *** p <0.001. n = 9 wells. (F and G) Box plots showing the relative spheroid area with integrin blocking antibodies in collagen I gel (F) and Matrigel (G). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Statistical comparisons were performed using the Kruskal‒Wallis test followed by Dunn’s post hoc test. Adjusted p -values from the Benjamini‒Hochberg method are shown: * p <0.05, ** p <0.01, *** p <0.001. n = 9 wells pooled from 3 independent experiments.
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    (A) Schematic of the spheroid invasion assay. DP spheroids, each composed of 300 <t>HFDPCs,</t> were placed on an ECM gel layer and overlaid with an additional ECM gel layer. The spheroids <t>were</t> <t>cultured</t> and observed every 12 hours. (B) Phase-contrast images of DP spheroids cultured in collagen I gel (top) and Matrigel (bottom). The colored squares indicate regions magnified in the bottom right images. Scale bar: 100 µm. (C) Binary-masked images highlighting the spheroid area. (D) Line graphs showing the changes in spheroid area over time in collagen I gel (red) and Matrigel (green). (E) Box plots showing the relative spheroid area at 12 h, normalized to the initial time point (1 h). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Two-tailed unpaired t -tests were used: * p <0.05, ** p <0.01, and *** p <0.001. n = 9 wells. (F and G) Box plots showing the relative spheroid area with integrin blocking antibodies in collagen I gel (F) and Matrigel (G). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Statistical comparisons were performed using the Kruskal‒Wallis test followed by Dunn’s post hoc test. Adjusted p -values from the Benjamini‒Hochberg method are shown: * p <0.05, ** p <0.01, *** p <0.001. n = 9 wells pooled from 3 independent experiments.
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    (A) Schematic of the spheroid invasion assay. DP spheroids, each composed of 300 <t>HFDPCs,</t> were placed on an ECM gel layer and overlaid with an additional ECM gel layer. The spheroids <t>were</t> <t>cultured</t> and observed every 12 hours. (B) Phase-contrast images of DP spheroids cultured in collagen I gel (top) and Matrigel (bottom). The colored squares indicate regions magnified in the bottom right images. Scale bar: 100 µm. (C) Binary-masked images highlighting the spheroid area. (D) Line graphs showing the changes in spheroid area over time in collagen I gel (red) and Matrigel (green). (E) Box plots showing the relative spheroid area at 12 h, normalized to the initial time point (1 h). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Two-tailed unpaired t -tests were used: * p <0.05, ** p <0.01, and *** p <0.001. n = 9 wells. (F and G) Box plots showing the relative spheroid area with integrin blocking antibodies in collagen I gel (F) and Matrigel (G). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Statistical comparisons were performed using the Kruskal‒Wallis test followed by Dunn’s post hoc test. Adjusted p -values from the Benjamini‒Hochberg method are shown: * p <0.05, ** p <0.01, *** p <0.001. n = 9 wells pooled from 3 independent experiments.
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    Image Search Results


    (A) Schematic of the spheroid invasion assay. DP spheroids, each composed of 300 HFDPCs, were placed on an ECM gel layer and overlaid with an additional ECM gel layer. The spheroids were cultured and observed every 12 hours. (B) Phase-contrast images of DP spheroids cultured in collagen I gel (top) and Matrigel (bottom). The colored squares indicate regions magnified in the bottom right images. Scale bar: 100 µm. (C) Binary-masked images highlighting the spheroid area. (D) Line graphs showing the changes in spheroid area over time in collagen I gel (red) and Matrigel (green). (E) Box plots showing the relative spheroid area at 12 h, normalized to the initial time point (1 h). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Two-tailed unpaired t -tests were used: * p <0.05, ** p <0.01, and *** p <0.001. n = 9 wells. (F and G) Box plots showing the relative spheroid area with integrin blocking antibodies in collagen I gel (F) and Matrigel (G). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Statistical comparisons were performed using the Kruskal‒Wallis test followed by Dunn’s post hoc test. Adjusted p -values from the Benjamini‒Hochberg method are shown: * p <0.05, ** p <0.01, *** p <0.001. n = 9 wells pooled from 3 independent experiments.

    Journal: bioRxiv

    Article Title: Basement membrane components define the microenvironment of aggregated fibroblasts in the skin and support their aggregation in vitro

    doi: 10.1101/2025.08.27.672757

    Figure Lengend Snippet: (A) Schematic of the spheroid invasion assay. DP spheroids, each composed of 300 HFDPCs, were placed on an ECM gel layer and overlaid with an additional ECM gel layer. The spheroids were cultured and observed every 12 hours. (B) Phase-contrast images of DP spheroids cultured in collagen I gel (top) and Matrigel (bottom). The colored squares indicate regions magnified in the bottom right images. Scale bar: 100 µm. (C) Binary-masked images highlighting the spheroid area. (D) Line graphs showing the changes in spheroid area over time in collagen I gel (red) and Matrigel (green). (E) Box plots showing the relative spheroid area at 12 h, normalized to the initial time point (1 h). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Two-tailed unpaired t -tests were used: * p <0.05, ** p <0.01, and *** p <0.001. n = 9 wells. (F and G) Box plots showing the relative spheroid area with integrin blocking antibodies in collagen I gel (F) and Matrigel (G). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Statistical comparisons were performed using the Kruskal‒Wallis test followed by Dunn’s post hoc test. Adjusted p -values from the Benjamini‒Hochberg method are shown: * p <0.05, ** p <0.01, *** p <0.001. n = 9 wells pooled from 3 independent experiments.

    Article Snippet: Primary human follicle DP cells (HFDPCs) were purchased from PromoCell (Heidelberg, Germany) and cultured in Follicle Papillae Growth Medium (PromoCell) at 37°C, 5% CO 2 and 100% humidity.

    Techniques: Invasion Assay, Cell Culture, Two Tailed Test, Blocking Assay